http://www4.od.nih.gov/oba/rac/guidelines_02/APPENDIX_B.htm
Appendix B-I. Risk Group 1 (RG1) Agents
RG1 agents are not associated with disease in healthy adult humans. Examples of RG1 agents include asporogenic
Bacillus subtilis or Bacillus licheniformis (see Appendix C-IV-A, Bacillus subtilis or Bacillus licheniformis Host-Vector
Systems, Exceptions); adeno- associated virus (AAV) types 1 through 4; and recombinant AAV constructs, in which the
transgene does not encode either a potentially tumorigenic gene product or a toxin molecule and are produced in the
absence of a helper virus. A strain of Escherichia coli (see Appendix C-II-A, Escherichia coli K-12 Host Vector Systems,
Exceptions) is an RG1 agent if it (1) does not possess a complete lipopolysaccharide (i.e., lacks the O antigen); and (2)
does not carry any active virulence factor (e.g., toxins) or colonization factors and does not carry any genes encoding
these factors.
Appendix B-V. Animal Viral Etiologic Agents in Common Use
The following list of animal etiologic agents is appended to the list of human etiologic agents. None of these agents is
associated with disease in healthy adult humans; they are commonly used in laboratory experimental work.
A containment level appropriate for RG1 human agents is recommended for their use. For agents that are
infectious to human cells, e.g., amphotropic and xenotropic strains of murine leukemia virus, a containment
level appropriate for RG2 human agents is recommended.
Baculoviruses
Herpesviruses
--Herpesvirus ateles
--Herpesvirus saimiri
--Marek's disease virus
--Murine cytomegalovirus
Papovaviruses
--Bovine papilloma virus
--Polyoma virus
--Shope papilloma virus
--Simian virus 40 (SV40)
Retroviruses
--Avian leukosis virus
--Avian sarcoma virus
--Bovine leukemia virus
--Feline leukemia virus
--Feline sarcoma virus
--Gibbon leukemia virus
--Mason-Pfizer monkey virus
--Mouse mammary tumor virus
--Murine leukemia virus
--Murine sarcoma virus
--Rat leukemia virus