Sphingomonas strains from bioremediation to human disease

Osteomyelitis and Secondary Septic Arthritis Caused by ...Human infection by Sphingomonas paucimobilis is rare. We
present a case of osteomyelitis and septic arthritis in an. immunocompromised patient in which ...
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Recurrent Sphingomonas paucimobilis- bacteraemia associated with a ...2002 The Hospital Infection Society.
Keywords: Sphingomonas; bacteraemia; nosocomial infection; random amplified polymorphic DNA. technique.
Introduction ...
linkinghub.elsevier.com/retrieve/pii/S0195670101911636 - Similar pages

Blackwell Synergy - Transplant Infect Dis, OnlineEarly Articles ...Osteomyelitis and secondary septic arthritis caused
by Sphingomonas paucimobilis. Infection 2005; 33: 93–95. CrossRef, Medline, ISI ...
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CAT.INISTHemopathy ; Leukopenia ; Bacteria ; Infection ; Bacteriosis ; Case study ; Microbiological investigation ;
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Nosocomial Infections Caused by Sphingomonas paucimobilis ...From January 1995 to September 1996, 14 isolates of
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Applied and Environmental Microbiology, April 2004, p. 1944-1955, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.1944-1955.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Occurrence and Phylogenetic Diversity of Sphingomonas Strains in Soils Contaminated with Polycyclic Aromatic
Hydrocarbons
Natalie M. E. J. Leys,1,2, Annemie Ryngaert,1 Leen Bastiaens,1 Willy Verstraete,2 Eva M. Top,2, and Dirk Springael1,
3*
Environmental Technology, Flemish Institute for Technological Research, 2400 Mol,1 Laboratory of Microbial Ecology
and Technology, University of Ghent, 9000 Ghent,2 Laboratory for Soil and Water Management, Catholic University of
Leuven, 3001 Heverlee, Belgium3

Received 3 September 2003/ Accepted 5 December 2003

Bacterial strains of the genus Sphingomonas are often isolated from contaminated soils for their ability to use
polycyclic aromatic hydrocarbons (PAH) as the sole source of carbon and energy. The direct detection of
Sphingomonas strains in contaminated soils, either indigenous or inoculated, is, as such, of interest for bioremediation
purposes. In this study, a culture-independent PCR-based detection method using specific primers targeting the
Sphingomonas 16S rRNA gene combined with denaturing gradient gel electrophoresis (DGGE) was developed to
assess Sphingomonas diversity in PAH-contaminated soils. PCR using the new primer pair on a set of template DNAs
of different bacterial genera showed that the method was selective for bacteria belonging to the family
Sphingomonadaceae. Single-band DGGE profiles were obtained for most Sphingomonas strains tested. Strains
belonging to the same species had identical DGGE fingerprints, and in most cases, these fingerprints were typical for
one species. Inoculated strains could be detected at a cell concentration of 104 CFU g of soil–1. The analysis of
Sphingomonas population structures of several PAH-contaminated soils by the new PCR-DGGE method revealed that
soils containing the highest phenanthrene concentrations showed the lowest Sphingomonas diversity. Sequence
analysis of cloned PCR products amplified from soil DNA revealed new 16S rRNA gene Sphingomonas sequences
significantly different from sequences from known cultivated isolates (i.e., sequences from environmental clones
grouped phylogenetically with other environmental clone sequences available on the web and that possibly originated
from several potential new species). In conclusion, the newly designed Sphingomonas-specific PCR-DGGE detection
technique successfully analyzed the Sphingomonas communities from polluted soils at the species level and revealed
different Sphingomonas members not previously detected by culture-dependent detection techniques.